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The above-mentioned ex vivo staining means hinges on the availability of feasible muscle regarding magnitude of around 20 million each day part. Small needle biopsies depict such as scarce, but clinically interesting procedure. We had the opportunity to use samples of the brand new Amsterdam LN biopsy cohort for the analysis , and in which framework used tissues that had been in the vitro propagated just before cryopreservation and you may tetramer staining. Nonetheless, i been able to come across citrulline-activated T tissue various specificities in every the brand new RA people plus in some of the arthralgia people. We including included some LN-related phenotypic indicators, concentrating on PD-step one, CXCR5 and ICOS, in order to ask the current presence of a traditional follicular T assistant phenotype . (más…)